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Nuchal Ligament Biopsy

A new proceedure for tissue diagnosis is being tested for a possible early DSLD/ESAP detection method. This is a biopsy of nuchal ligament tissue taken from a live horse. Several horses have had the biopsy and have shown findings consistant with tissue diagnosis done at necropsy. If you would like to test your horse please have your vet contact Dr Halper for instructions and tissue handling before proceedure.

The proceedure should only take approximately ten minutes to perform. Horse is sedated for proceedure and then sutured after biopsy. Tissues frozen and shipped overnight.

Contact Dr Halper at:

Tissue Diagnosis of DSLD

Dr. Jaroslava Halper
Department of Pathology
College of Veterinary Medicine
The University of Georgia
Athens, GA 30602-7388
Phone: 1-706-542-5830
Fax: 1-706-542-5828
e-mail: jhalper@vet.uga.edu

Our laboratory will accept tissues from horses known or suspected to have DSLD. Though there is no definitive diagnostic test for DSLD available at the present time, our testing will provide you with positive results consistent with the presence of DSLD in your horse. Negative results mean that in all likelihood the examined horse did not have DSLD. The more tissues we can get, the more accurate our testing will be. This will also increase our capability to assess the extent and severity of the disease.

Hematoxylin & eosin is the basic stain in histology. We usually do alcian blue (special stain) to show that the material accumulated in tissues is proteoglycan.

Partial evaluation: Depends on number of slides
H&E slide...................................$--6
Special stain at least......................$ 20
Evaluation..................................$ 50

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