In Amazing Spider-Man #510, Peter receives a letter from his assailants in which they threaten to kill Mary Jane and Aunt May. Not wanting to alarm his wife and aunt, Peter remains silent about the content of the letter. Later, as Spider-Man, Peter pays a visit to Detective Lamont to get help in obtaining some latent handwriting impressions from Gwen's letter. After leaving Lamont, he calls his aunt to check on her. A male voice answers the phone and tells him that he has to make his way to a certain address in less than ten minutes or else Aunt May dies. Peter/Spider-Man web-slings as fast as he can and reaches the destination. As he approaches what appears to be Aunt May tied to a chair in the middle of the factory, his spider-sense goes ballistic and he realizes that it is a trap. He escapes a huge explosion that completely destroys the factory but he is partially caught in the blast. As he lies in the rubble of the factory, bruised and battered, the male assailant shows up and starts beating him to a bloody pulp. The intervention of the female assailant saves his life. The male assailant, however, vows to return and finish what he has started. Their names are revealed - the woman is named Sarah, the man Gabriel. Later that night, Peter/Spider-Man meets once again with Detective Lamont. The letter reveals that Gwen was pregnant when she left for Europe and that she had twins - their names: Gabriel and Sarah.

ACT 1: Peter reads the letter over and over again and cannot make sense of it. He knows that it is impossible for them to be Gwen's kids because if it were true, they would still be children; unless they weren't born normal kids to begin with. He makes his way back to his apartment and finds MJ sleeping (well, pretending to sleep anyways). She awakens (again pretending to wake up) and immediately perceives that something is wrong with Peter. Peter, however, remains silent and does not reveal to MJ what he's just found out. He heads into the kitchen to prepare breakfast, while MJ seems to have some kind of internal struggle about keeping people's secrets.

ACT 2: Later that day, Peter heads back to the cemetery to visit Gwen's grave once more. Unbeknownst to him, he is being surveyed by Gabriel. Strangely, Peter suddenly drives some kind of long stick into the ground just above Gwen's coffin. Using a walkie-talkie, Gabriel radios his sister to tell her that Peter is "confirming his worst nightmare" and that "if he didn't know before, he knows now". The story switches over to Mary Jane who is at a practice for her off-Broadway play. The other actors ask her to join them at one of their places to go over a few more scenes. Mary Jane politely refuses, saying that she's got something on her mind that she needs to take care of. She heads back to the apartment and looks in the closet where she saw Peter hide something up there earlier that morning. She finds the sheets with the results of the latent handwriting impression testing.

ACT 3: Peter, as Spider-Man, heads off to a Genetic Lab to perform some DNA testing using the letters sent to him. Peter knows that an amateur would use gloves to make sure no fingerprints on the envelope, forgetting that when he licks the envelope, he leaves behind more than enough traces of DNA for a positive identification or cross-match. Using DNA obtained from his visit to Gwen's grave (yes, that's what that long stick was for!), Peter performs a few tests to determine whether or not Gabriel and Sarah are indeed Gwen's kids. Suddenly however, Sarah, the female assailant, confronts him, with a gun pointing in his direction. She tells Spidey that her brother doesn't want to just kill him but to make him suffer by killing everyone he loves. Spidey asks Sarah if she wants the same thing as her brother. For a second, Sarah appears to hesitate before saying that the only way to save the people he cares about is to willingly let her kill him right there, right now. At that exact moment, the bell of the DNA testing machine rings, which startles Sarah and catches her off guard. Spider-Man uses that opportunity to leap in her direction and disarm her. When he pulls her mask off, he is shocked to see that she looks exactly like Gwen Stacy. Suddenly, his spider-sense goes wild and a nearby window blows up, sending pieces of glass all over the place. Spidey dives out of harm's way. He turns around to see if Sarah is all right but she is nowhere to be found. He checks on the results of the DNA test, which confirm that Sarah and Gabriel are indeed the children of Gwen Stacy. Meanwhile, on the rooftop of a nearby building, Sarah reunites with her brother. She tells him that Peter/Spider-Man saw her face and that he reacted just as they had thought he would. Gabriel says that it just confirms what they already knew and what Peter was confirming for himself up in the lab; that he is their father, that he abandoned them, deserted their mother and later caused her death.

ACT 4: Later that night, Peter returns to his apartment. Mary Jane is waiting for him and tells him that she found the letter and read it. Peter replies that it can't be his kids because Gwen and him never slept together. MJ knows that Peter is telling the truth because she knows who the real father of Gwen Stacy's children is. To be continued.