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DNA Application Answers

   DNA Application Review Answers:

     1. D - Both Transformation and DNA Fingerprinting require the use of restriction enzymes. DNA Sequencing does not use restriction enzymes. Rather, this application uses enzymes that destroy certain bases of DNA, instead of simply cutting the DNA at recognition sequences.

     2. C - DNA Fingerprinting has applications to Forensic Science and is used constantly to determine who is the criminal.

     3. A- Transformation uses bacteria to which plasmids are added.

     4. B - DNA Sequencing allows for genes in individuals to be identified.

     5. D - Transformation involves the addition of plasmid DNA to a bacterium. DNA Sequencing involves the destruction of certain nucleotides.

     6. B - DNA Sequencing is used in the Human Genome Project in an attempt to uncover the nucleotide arrangement in the entire human genome.

     7. F - The lac operon is the structure, not technique, by which bacteria have the ability to produce certain proteins capable of catabolizing lactose.

     8. C - DNA Fingerprinting is the application used to determine paternity of a child.

     9. D - Both DNA Fingerprinting and DNA Sequencing use the technique of Gel Electrophoresis to separate DNA segments by size in an agarose matrix.

     10. A - Transformation involves the addition of plasmids to bacteria.

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