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Beschrijving: Chemistry - Biology
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VERNIER SOFTWARE 
CHEMISTRY AND BIOLOGY PROBES 
WITH THE TI CBL AND TI-82, TI-83, TI-85, TI-86, AND TI-92
   I. OVERVIEW
  II. MEMORY REQUIREMENTS AND MAXIMUM DATA
 III. GROUP FILES
  IV. SENDING GROUP FILES - MAC
   V. SENDING GROUP FILES - WINDOWS
  VI. UNGROUPING FILES
 VII. PROTECTED PROGRAM FILES
VIII. PERFORMING EXPERIMENTS WITH CHEMBIO
  IX. USING THE HEART RATE MONITOR
   X. USING THE VERNIER EKG SENSOR
  XI. OVERVIEW OF EACH PROGRAM
 XII. PROGRAM DESIGN NOTES
 
I. OVERVIEW
This document describes the use of a group of programs that 
support Vernier Software chemistry and biology probes for use 
with a TI calculator and the TI Calculator-Based Laboratory (CBL) 
System. The following Vernier probes are directly supported: 
Vernier Direct-Connect Temperature Probe, pH, Pressure Sensor, 
Colorimeter, Voltage Measurement Leads, Conductivity Probe, 
Dissolved Oxygen Probe, Barometer, Relative Humidity Sensor, 
Biology Gas Pressure Sensor, Respiration Monitor Belt, EKG 
Sensor, Heart Rate Monitor, Exercise Heart Rate Monitor, Standard 
Temperature Probe, Quick Response Temperature Probe, and 
Thermocouple. The Texas Instruments Temperature Probe, Voltage 
Probe, and Light Probe are also supported. 
 
II. MEMORY REQUIREMENTS
This entire set of programs may require more than half the 
available memory on the calculator If you run into memory errors, 
it may be necessary to delete one or more programs from the 
calculator. If there are certain data collection modes you are 
not using, e.g. Trigger, you can delete the CMBTRIGG program 
associated with this mode. (You will find a description of each 
program later in this document.) You may also want to consider 
deleting the CMBHEART and CMBEKG programs if you are not using 
the Heart Rate Monitor or EKG sensor.
If you reset your calculator, then load the CHEM-BIO group file, 
the maximum number of point you can collect with all three probes 
on the TI-82 is 99, the TI-83 300, the TI-85 100, the TI-86 512, 
and the TI-92 370.
III. GROUP FILES
The CHEM-BIO group file is twelve programs that have been grouped 
together. Groups of files for the TI calculator always have a 
"??g" suffix where "??" represents the various calculators: 82, 
83, 85, 86, or 92. Individual program files have the extensions 
"??p" The extensions on the filenames appear only on the computer 
and do not appear when you see the program names on the 
calculator. The easiest method of loading all twelve programs on 
to your calculator is to transfer the CHEM-BIO.??g group file.
IV. SENDING GROUP FILES - MAC
TI-82
1) Connect the TI-Graph Link cable to the modem port of the 
Macintosh computer and to the port on the bottom edge of the 
TI-82.
2) On the TI-82 calculator, use LINK, then select Receive 
("Waiting..." appears on the TI-82 screen.
3) Using the TI-Graph Link software on your computer, choose 
Group from the Send menu.
4) Open the CHEM-BIO.82g file from the TI-82 folder in your 
Vernier CBL Data Collection disk (or on your computer hard 
drive).
5) Click on the Send button. The twelve programs are now loaded 
into your TI-82 calculator. 
TI-83
1) Connect the TI-GRAPH LINK cable to the modem port of the 
Macintosh computer and to the port on the bottom edge of the TI-
83.
2) Turn on the TI-83 Graphing calculator.
3) Using the TI-GRAPH LINK software on your computer, choose Send 
from the Link menu. Then choose Group from the Select pull-down 
menu near the bottom of this window.
4) Open the CHEM-BIO.83g file from the TI-83 folder in your 
Vernier CBL Data Collection disk (or on your computer hard 
drive). Then click on Add.
5) Click on the Send button. The twelve programs are now loaded 
into your TI-83 calculator. 
TI-85
1) Connect the TI-GRAPH LINK cable to the modem port of the 
Macintosh computer and to the port on the bottom edge of the TI-
85.
2) On the TI-85 calculator, use LINK, then select Receive 
("Waiting..." appears on the TI-85 screen.
3) Using the TI-GRAPH LINK software on your computer, choose 
Group from the Send menu.
4) Open CHEM-BIO.85g from the TI-85 folder in your Vernier CBL 
Data Collection disk (or on your computer hard drive).
5) Click on the Send button. The twelve programs are now loaded 
into your TI-85 calculator. 
TI-92
1) Connect the TI-GRAPH LINK cable to the modem port of the 
Macintosh computer and to the port on the top edge of the TI-92.
2) Using the TI-GRAPH LINK software on your computer, choose Send 
from the Link menu.
3) Change the Select field at the bottom of the send screen from 
All Objects to Groups.
4) Open the CHEM-BIO.92g file from the TI-92 folder in your 
Vernier CBL Data Collection disk (or on your computer hard 
drive).
5) Select the CHEM-BIO.92g file and click on the Add button.
The file will be added to the bottom window. 
6) Click on the Send button. The twelve programs are now loaded 
into your TI-92 calculator. 
V. SENDING GROUP FILES - WINDOWS
TI-82
1) Connect the TI-Graph Link cable to the serial port of your 
computer and to the port on the bottom edge of the TI-82.
2) On the TI-82 calculator, use LINK, then select Receive 
("Waiting..." appears on the TI-82 screen.
3) Using the TI-Graph Link software on your computer, choose Send 
from the Link menu.
4) Select CHEM-BIO.82g from the TI-82 folder in your Vernier CBL 
Data Collection disk (or on your computer hard drive).
5) Click on the OK button. The twelve programs are now loaded 
into your TI-82 calculator. 
TI-83
1) Connect the TI-GRAPH LINK cable to the serial port of your 
computer and to the port on the bottom edge of the TI-83.
2) Turn the calculator on.
3) In TI-GRAPH LINK, choose Send from the Link menu.
4) Select CHEM-BIO.83g from the TI-83 folder in your Vernier CBL 
Data Collection disk (or on your computer hard drive).
5) Click on the add button then select OK. The twelve programs 
are now loaded into your TI-83 calculator. 
TI-85
1) Connect the TI-GRAPH LINK cable to the serial port of your 
computer and to the port on the bottom edge of the TI-85.
2) On the TI-85 calculator, use LINK, then select Receive 
("Waiting..." appears on the TI-85 screen.
3) Using the TI-GRAPH LINK software on your computer, choose Send 
from the Link menu.
4) Select CHEM-BIO.85g from the TI-85 folder in your Vernier CBL 
Data Collection disk (or on your computer hard drive).
5) Click on the OK button. The twelve programs are now loaded 
into your TI-85 calculator. 
TI-86
1) Connect the TI-GRAPH LINK cable to the serial port of your 
computer and to the port on the bottom edge of the TI-86.
2) Turn the calculator on.
3) In TI-GRAPH LINK, choose Send from the Link menu.
4) Select CHEM-BIO.86g from the TI-86 folder in your Vernier CBL 
Data Collection disk (or on your computer hard drive).
5) Click on the add button then select OK. The twelve programs 
are now loaded into your TI-86 calculator. 
TI-92
1) Connect the TI-GRAPH LINK cable to the serial port of your 
computer and to the port on the top edge of the TI-92.
2) Using the TI-GRAPH LINK software on your computer, choose Send 
from the Link menu.
3) Select PHYSICST.92g from the TI-92 folder in your Vernier CBL 
Data Collection disk (or on your computer hard drive).
4) Click on the Add button and the file will be copied to the 
bottom send window.
5) Click on the OK button. The twelve programs are now loaded 
into your TI-92 calculator. 
VI. UNGROUPING FILES 
 
You may prefer to "ungroup" the CHEM-BIO.??g file for archiving 
on a hard drive. Before you do that, it would be best to create a 
folder or subdirectory on your hard drive for the group file. The 
programs can then be stored in a common area on your hard drive 
after being ungrouped. The files can be ungrouped by choosing the 
Ungroup Files option in the TI-GRAPH LINK program. Ungrouping the 
file will make the following twelve programs available: CHEMBIO, 
CMBCALIB, CMBCALS, CMBDERIV, CMBEKG, CMBGRAPH, CMBHEART, 
CMBMONIT, CMBREGRS, CMBTIMEG, CMBTRIGP, and CMBTRIGG. The 
TI-GRAPH LINK will also allow you to download all thirteen 
programs from the computer to the calculator. 
VII. PROTECTED PROGRAM FILES
The calculator programs described, except for the TI-85, have 
been protected so that they cannot be edited on the calculator. 
This feature prevents students from accidentally corrupting a 
program. The easiest way to edit protected files is with the TI-
GRAPH LINK program. The TI-GRAPH LINK manuals describe the 
protecting and unprotecting process.
VIII. PERFORMING EXPERIMENTS WITH THE CHEMBIO PROGRAM 
 
The CHEMBIO programs function together to provide a wide range of 
options as you create and run experiments. After all twelve 
programs have been loaded, run the CHEMBIO program. The CHEMBIO 
program uses a series of menus to set up different types of 
experiments with a combination of the probes described in the 
following section. 
 
The following general description applies to the use of these 
programs on the calculator. For specific help in executing 
programs on the calculator, refer to the manual that came with 
the calculutor. 
 
To begin, turn on the CBL and link the Calculator and CBL with 
the black cord that came with the calculator. Make sure that the 
plugs are firmly pushed into the I/O port on the bottom edge (or 
the top edge on the TI-92) of the calculator and CBL. Connect the 
appropriate probes starting with Channel 1 (CH1) found on the top 
edge of the CBL. On the calculator run the CHEMBIO program. After 
an introductory screen, the following MAIN MENU will appear: 
 
***MAIN MENU*** 
1:SET UP PROBES 
2:COLLECT DATA 
3:VIEW GRAPH 
4:VIEW DATA
5:FIT CURVE
6:RETRIEVE DATA 
7:QUIT 
*****************************************************************
TI-85 and TI-86 users will see slightly different menus because 
the TI-85 and TI-86 menus are limited to 5 elements in a menu, 
also the menu items can only have 5 characters. The MAIN MENU on 
the TI-85 or TI-86 will be:
***MAIN MENU***
SETUP=SET UP PROBES 
COLL =COLLECT DATA 
VIEW =VIEW GRAPH
DATA =VIEW DATA
MORE =MORE
The MORE option will bring up this menu:
FIT C=FIT CURVE
RETRI=RETRIEVE DATA
QUIT =QUIT 
RETRN=RETURN
The RETRN option will bring you back to the first part of the 
MAIN MENU. When selecting MORE be sure to use the [F5] key, not 
the [MORE] key on the calculator. NOTE: the menus in the 
following description will match the TI-82, TI-83, and TI-92. The 
TI-85 and TI-86 menus will differ slightly.
*****************************************************************
The first option, SET UP PROBES, is used to 1) enter the number 
of active probes, 2) choose a probe, 3) enter the CBL channel 
number for the probe, and 4) choose the method of calibrating 
Vernier probes. After you select this option, the calculator will 
attempt to communicate with the CBL. If the CBL is not powered up 
or the CBL and calculator are not properly linked, an error 
message will appear. Check to see that the link cord is firmly 
connected to each device and that the CBL is powered up - press 
the [ON/HALT] button on the CBL. Correct any problems before 
continuing. The calculator will then ask you to enter the number 
of probes and then the following menu will appear:
 
SELECT PROBE 
1:TEMPERATURE 
2:PH 
3:PRESSURE 
4:COLORIMETER 
5:VOLTAGE 
6:CONDUCTIVITY 
7:MORE PROBES 
 
These options represent the following Vernier Software probes: 
Vernier Direct-Connect Temperature Probe, pH System, Pressure 
Sensor, Colorimeter, Voltage Measurement Leads, and Conductivity 
Probe. The option TEMPERATURE and can be used either with a 
Direct-Connect temperature probe or with the TI temperature probe 
that comes with the CBL. The option VOLTAGE can be used for the 
Vernier Voltage Probe and TI Voltage Probe that come with the 
CBL. If you select the option MORE PROBES, you will get another 
list of probes: 
 
SELECT PROBE 
1:D.OXYGEN 
2:BAROMETER 
3:LIGHT 
4:REL HUMID 
5:BIO PRESSURE 
6:HEART RATE 
7:MORE PROBES 
 
The first six options represent the following Vernier probes: 
Dissolved Oxygen Sensor, Barometer, Light Sensor, Relative 
Humidity, Biology Gas Pressure Sensor, and Heart Rate Monitor. 
The option LIGHT can be used for the Vernier Light Sensor or the 
TI Light Probe that came with the CBL. If you select the option 
MORE PROBES, you will get another list of probes: 
 
SELECT PROBE 
1:VERNR STD TEMP 
2:VERNR QIK TEMP 
3:THERMOCOUPLE 
4:RESPIR. BELT 
5:EKG 
6:C02 SENSOR
7:RETURN 
 
These options represent the following Vernier probes: Standard 
Temperature Probe (can also be used with the Extra Long 
Temperature Probe), Quick-Response Temperature Probe, 
Thermocouple, Respiration Monitor Belt, EKG Sensor, and the CO2 
Sensor. The RETURN option returns you to the first list of 
probes. 
 
The above screen(s) will appear up to three times, depending upon 
the number of probes you entered, except in the case of the Heart 
Rate Monitor and EKG. (These two options run separate programs 
described later.) After you select a probe, you will be asked to 
enter the channel number for that probe. This program assumes 
that the probes are connected to the lowest available channels.
 
NOTE: It is important to mention another consideration when 
setting up an experiment with some probes. Certain combinations 
of probes will interact with each other if they are put in the 
same solution and connected to the same CBL. Do not use 
combinations of the following probes on the same CBL and in the 
same solution: Dissolved Oxygen Probe and Conductivity Probe with 
each other or in combination with the pH System or Vernier 
Direct-Connect Temperature Probe. The calculator will warn you if 
a combination will not work together.
 
After you enter the channel number for a Vernier probe, you will 
be presented with a CALIBRATION menu, except in the case of the 
Colorimeter. (The Colorimeter should be calibrated each time it 
is used. Therefore, when you select that probe, you will be sent 
to the PERFORM NEW calibration option described below.) The 
following is the CALIBRATION menu for the other Vernier probes: 
 
**CALIBRATION** 
1:USE STORED 
2:PERFORM NEW 
3:MANUAL ENTRY
(when using a Dissolved Oxygen Probe, a fourth option appears, 
4:POLARIZE PROBE) 
 
Option 1, USE STORED, allows you to load calibration values for 
the various Vernier probes from a calculator file. These are 
"generic" calibrations determined by Vernier Software. The values 
will provide reasonably accurate measurements. However, 
individual probes differ and you may obtain better results by 
performing a new calibration. The CMBCALS program contains these 
slope and intercept values. It is possible to edit this program 
and enter the slope and intercept values for your probes.
Option 2, PERFORM NEW, is used to perform a new calibration. To 
understand the calibration process, it is helpful to understand 
the operation of Vernier probes. During their operation, the 
probes produce a voltage that is linearly dependent upon a 
quantity being measured. For example, the temperature probe 
produces a voltage that varies linearly with the surrounding 
temperature. During the calibration process, the computer 
establishes the linear relationship between voltage and 
temperature. Choosing the PERFORM NEW option will allow you to 
perform a two-point calibration for the probe that was just set 
up. The following paragraph describes the calibration process for 
a temperature probe. The same general procedure can apply to most 
of our probes. 
 
Begin by placing the temperature probe in an ice bath. As the 
probe is swirled in the ice water, monitor the CBL to see the 
voltage produced by the probe. To monitor other channels, press 
the [CH VIEW] button on the CBL. As you repeatedly press and hold 
this button, you will be able to cycle through all the channels. 
You will be able to see which channel is currently being 
monitored by noting the blinking CH 1, CH 2, or CH 3 symbols in 
the upper left corner of the CBL display. When the voltage 
stabilizes, press the [TRIGGER] button on the CBL. When the 
voltage stabilizes, press the [TRIGGER] button on the CBL. You 
are then prompted to enter the reference value, "0" (degrees 
Celsius). Place the temperature probe in a second reference such 
as boiling water or room temperature water. The voltage is again 
monitored and the [TRIGGER] button is pressed when the voltage 
stabilizes. Enter the second reference value measured with a 
thermometer. A slope and intercept for the linear calibration 
curve are then displayed on the calculator and automatically 
loaded into the CBL. You may want to record these values for 
future reference (see the section below). This process can be 
repeated for other Vernier probes. For help with a particular 
probe, refer to the information sheet for that probe. 
 
Option 3, MANUAL ENTRY, in the CALIBRATION menu is an option that 
may save you time in setting up future experiments, especially if 
you have performed calibrations for your probes. Probes such as 
temperature hold their calibration for long time periods. If you 
know the slope and intercept values determined in the above 
process, you can manually enter these values with this option. 
Some probes such as pH and Colorimeter do not hold their 
calibrations and may need to be recalibrated prior to their use. 
 
Option 4, POLARIZE PROBE, is used to polarize the Dissolved 
Oxygen Probe. When selected, a power-up command is sent to the 
CBL. The polarizing process takes thirty minutes and is necessary 
for proper operation of the probe. 
 
From the MAIN MENU you can set up an experiment by choosing the 
second option, COLLECT DATA. The following menu provides you with 
a choice of data-collection modes.
 
DATA COLLECTION 
1:MONITOR INPUT 
2:TIME GRAPH 
3:TRIGGER/PROMPT
4:TRIGGER
5:RETURN
 
The MONITOR INPUT option is used to monitor the active channels 
with the calculator and/or CBL. The purpose of this option is to 
view data at 1.0 second intervals. No data are stored. For most 
probe combinations, each active channel will be displayed on the 
calculator. However, if there is more than one active probe and 
the probe combination contains a pH System, Conductivity Probe, 
or Dissolved Oxygen Probe, you will only be allowed to view a 
single channel at a time and you will be prompted with the 
following menu to choose a channel: 
 
SELECT A CHANNEL 
1:CH 1 
2:CH 2 
3:CH 3 
4:QUIT 
 
After selecting a channel, the calculator will display a reading 
from that channel on the calculator except in the case of a 
Dissolved Oxygen Probe or pH system. In the case of these probes, 
you will have to monitor their readings on the CBL display. You 
will be presented with this message: 
 
MONITOR CBL 
DISPLAY. USE THE
[CH VIEW] BUTTON 
TO SELECT 
CHANNEL NUMBER X 
PRESS [TRIGGER] 
BUTTON TO QUIT. 
 
The CBL will be put in the "READY" mode and it will display the 
reading from channel 1. To monitor other channels, press the [CH 
VIEW] button on the CBL. As you repeatedly press and hold this 
button, you will be able to cycle through all the channels. You 
will be able to see which channel is currently being monitored by 
noting the blinking CH 1, CH 2, or CH 3 symbols in the upper left 
corner of the CBL display. When you are done monitoring the 
channels on the CBL, press the [TRIGGER] button on the CBL and 
select the QUIT option on the calculator. If you are monitoring 
the channels on the calculator screen, press the [+] key on the 
calculator to quit.
 
The TIME GRAPH option from the DATA COLLECTION menu is used to 
graph up to three channels as a function of time. The design of 
this option was based on the assumption that most chemistry and 
biology users would want a real-time or live display as data are 
being collected. In most cases this type of graph is possible but 
in some experiments a live display will not be available. A live 
display will be produced for either of the following situations:
1) Only one probe is active and the time between samples is less 
than 270 s.
	
2) When more than one probe of the same type is active; the 
sample time is greater than 1.00 s for two probes, or greater 
than 1.25 s for three probes; and the time between samples is 
less than 270 s. 
Data will not be displayed live in any of the following 
arrangements:
1) Two or more different probes are being used.
2) The sample time is less than 1.00 s for two probes or 1.25 s 
for three probes.
3) The sample time is greater than 270 s.
After you select the TIME GRAPH option, you will be prompted to 
enter the time between samples which is entered in seconds. If 
you want a live display, you must take into account the above 
criteria and enter an appropriate number. 
If you anticipate the data to be collected without a live 
display, the time between samples can be in the range of 0.000264 
to 0.2 s or 0.25 to 16000 s. (If you have three active probes, 
the minimum sample time is 0.000364 s.) After the sample time is 
entered, you will be prompted to enter the number of data points. 
The maximum number for the TI-82 is 99, for all others it is 512, 
but you may be limited by the available memory. (See section II 
for memory requirements.) The CBL will then be set up to collect 
data in either live or non-live display as described above. Note: 
When data are collected in a live display, you can stop data 
collection by pressing the [+] key.
In most experiments involving a time graph, data will be 
automatically sent from the CBL to the calculator. However, if 
the experiment is too long, the calculator will automatically 
power down (APD) and data will not be transferred to the 
calculator. This will happen in either of the following 
situations:
1) The time between samples is greater than 270 seconds.
2) Two or more different probes are being used and the total 
experiment is longer than 270 s.
In either case you will be prompted with a message saying that 
you will have to choose the RETRIEVE DATA option at the MAIN MENU 
at the end of the experiment.
 
After each experiment is run, the data are stored in lists on the 
calculator. The names of the lists are displayed on the 
calculator screen after data collection. In general the 
independent variable is stored in L1, channel 1 is L2, channel 2 
in L3, and channel 3 in L4.
 
The TRIGGER/PROMPT option is used to perform experiments in which 
the independent variable is entered from the calculator keyboard 
while the dependent variable is measured with the CBL probe or 
probes. For example, you could use a Pressure Sensor to monitor 
the pressure of a gas enclosed in a syringe as the volume 
changes. When the [TRIGGER] button on the CBL is pressed, the CBL 
measures the gas pressure and you will be prompted to enter the 
volume of the gas in the syringe. The independent variable data, 
volume in this case, is stored in list L1 and the dependent 
variable data is stored in list L2. If more than one probe is 
active, data is stored in lists L2 through L4.
Each time you press the [TRIGGER] button, you will get the 
following screen:
DATA COLLECTION
1:MORE DATA
2:STOP AND GRAPH
3:PAUSE
If you select option 1:MORE DATA, the CBL will be set up to make 
another reading. If you select option 2:STOP AND GRAPH, data 
collection will stop and a graph will be displayed. If you select 
option 3:PAUSE, the calculator will be put in a pause mode. When 
you are ready to collect more data, press [ENTER] on the 
calculator and follow the on-screen instructions. If the 
calculator has powered down due to the APD, turn on the 
calculator, then press the [ENTER] key.
The TRIGGER option allows the user to manually collect one sample 
at a time when the [TRIGGER] button on the CBL is pressed. Each 
time you press the [TRIGGER] button, you will get the following 
screen:
TRIGGER
1:MORE DATA
2:STOP
3:PAUSE
If you select option 1:MORE DATA, the CBL will be set up to make 
another reading. If you select option 2:STOP, data collection 
will stop and, if you have two active channels, a graph will be 
displayed. (The default graph in this case plots L1 as the 
independent variable and L2 as the dependent variable.) If you 
select option 3:PAUSE, the calculator will be put in a pause 
mode. When you are ready to collect more data, press [ENTER] on 
the calculator and follow the on-screen instructions. If the 
calculator has powered down due to the APD, turn on the 
calculator, then press the [ENTER] key. 
 
In either TRIGGER/PROMPT or TRIGGER you can monitor each active 
channel during data collection by pressing and holding the [CH 
VIEW] button on the CBL. Repeated pressing of this button will 
cycle you through the active channels. After you have collected 
data, you will be returned to the MAIN MENU.
The third option in the Main Menu, VIEW GRAPH, allows you to 
review previous graphs. As you view each graph, you will be able 
to use the left and right arrow buttons on the calculator to view 
the coordinates of each point. These graphs use the "ZoomStat" 
option to provide automatic scaling of axes. You may prefer to 
quit the program to set your own scaling. 
 
The fourth option, VIEW DATA, exits you from the program so you 
can view the data.
The fifth option in the MAIN MENU, FIT CURVE, is used to fit data 
in selected lists for linear or power regression. Linear and 
power regression statistics are calculated, then a regression 
curve is displayed on a graph. It is also possible to exit the 
program, perform a calculation on one of the original data lists, 
then return to the program and perform a linear or power 
regression using the new calculated list. Here is a list of 
regression and list options available in FIT CURVE:
REGRESSION/LIST
1:LINEAR L1,L2
2:LINEAR L1,L3
3:LINEAR L1,L4
4:LINEAR L3,L2
5:POWER L1,L2
6:POWER L1,L3
7:RETURN
The sixth option on the MAIN MENU, RETRIEVE DATA, is used after 
long-term data are collected without a live display. Before you 
select this option, be sure the CBL is done collecting data. (The 
word "DONE" should appear in the CBL display.) 
The seventh option, QUIT, in the MAIN MENU will exit you from the 
program and return you to the Home screen. 
 
IX. USING THE HEART RATE MONITOR
 
OVERVIEW 
 
The Vernier Software Heart Rate Monitor operates by monitoring 
the light level transmitted through the skin. A clip is attached 
to the ear lobe. One half of the clip contains an incandescent 
light bulb and the other half measures the amount of transmitted 
light. Since the intensity of the light is a function of the 
amount of blood in the ear, the output of the Heart Rate Monitor 
varies throughout the heart beat. When the light intensity data 
are collected as a function of time, a graph of intensity vs. 
time produces a periodic pattern. The pattern can be analyzed to 
determine the heart rate. 
The Exercise Heart Rate Monitor consists of a transmitter belt, 
plug-in receiver, and a large and small elastic strap. The 
transmitter belt is worn just below the chest and held in place 
by an elastic strap. The transmitter detects each heart beat 
through two electrodes with ECG accuracy and transmits the heart 
rate information to the plug-in receiver with the help of a low 
frequency electromagnetic field. The plug-in receiver wirelessly 
receives the transmission, and passes a 3-volt pulse for each 
heart beat detected to the CBL. The reception range of the plug-
in receiver in 80-100 cm or about 3 feet.
The following material briefly describes the CMBHEART program and 
its use with the Heart Rate Monitor, or Exercise Heart Rate 
Monitor, TI calculator, and the TI Calculator-Based Laboratory 
(CBL) System. The primary uses of the program are 1) to collect 
and display data produced by the Heart Rate Monitor 2) to 
calculate the heart rate in beats/minute and 3) store heart rate 
values in a list and graph them vs. time. This program can be run 
by itself or it will be run when you choose the Heart Rate 
Monitor from the SET UP PROBE option in the MAIN MENU of the 
CHEMBIO program. 
 
GENERAL DESCRIPTION OF THE PROGRAM
 
To begin, connect either heart rate monitor to channel 1 on the 
CBL. Attach the clip to the ear lobe, finger tip, or to the web 
of skin between the index finger and thumb, or attach the chest 
belt around your chest. Run the CMBHEART program or choose HEART 
RATE from the list of sensors in the SET UP PROBES option in 
CHEMBIO. After introductory screens, a menu will ask you whether 
you are using an ear clip or chest belt. Then the main menu will 
appear. 
 
***HEART RATE MENU***
1:COLLECT DATA 
2:VIEW LAST GRAPH 
3:VIEW BPM GRAPH 
4:QUIT 
 
To begin data collection, select option 1:COLLECT DATA. A graph 
will be displayed in the screen if you are using the Heart Rate 
Monitor. When data are retrieved from the CBL, time will be 
stored in list L1 and voltage will be stored in list L2. The data 
collected in L1 and L2 is used to draw a waveform which is 
displayed in the graph if you are using the Heart Rate Monitor. 
The calculated heart rate is displayed in beats per minute (BPM). 
The beats per minute for each sample is stored in L3. If the CBL 
and either heart rate monitor are unable to calculate the heart 
rate, a message saying "COLLECTING DATA PLEASE REMAIN STILL" will 
be displayed on the screen. If the calculator continues to 
display this message, you may need to move the earclip around, or 
move closer to the receiver until you find a spot that gives you 
a better reading. To stop data collection and return to the main 
menu, press [ENTER] on the calculator. 
 
The second option in the main menu, VIEW LAST GRAPH, displays a 
graph of the last data segment collected along with the 
calculated heart rate. This option is helpful when it is 
necessary to take a close look at the waveform or just to review 
what the heart rate was before data collection was stopped. To 
quit from this selection and return to the main menu, press 
[ENTER] on the calculator. 
 
The VIEW BPM GRAPH option takes the list of calculated heart 
rates (in BPM) stored in L3 and plots them vs. time. The length 
of time between samples is estimated to be 10 seconds. To read 
the points plotted on the graph press the right arrow direction 
key located at the top right hand corner of the calculator. This 
option is very useful if you are performing an experiment which 
involves calculating an individuals recovery time or the effect 
of miscellaneous stimuli on heart rate. To quit from this 
selection and return to the main menu, press [ENTER] on the 
calculator.
 
X. USING THE VERNIER EKG SENSOR 
OVERVIEW 
 
The following material briefly describes the CMBEKG program and 
its use with the EKG Sensor, TI Calculator, and the TI 
Calculator-Based Laboratory (CBL) System. The primary uses of the 
program are 1) to collect and display data produced by the EKG 
Sensor 2) graph the EKG data vs. time and allow the user to trace 
the data points for analysis of the EKG waveforms. This program 
can be run by itself or it will be run when you choose the EKG 
Sensor from the SET UP PROBE option in the MAIN MENU of the 
CHEMBIO program. 
 
GENERAL DESCRIPTION OF THE PROGRAM
 
To begin, connect the EKG Sensor to channel 1 on the CBL. Place 
the electrode tabs along the subject's arms or legs as described 
in the EKG manual. Connect the three sensor leads to the 
electrode tabs. Run the CMBEKG program or choose EKG from the 
list of probes. The following main menu will appear. 
 
CHEM-BIO EKG 
1:COLLECT DATA 
2:VIEW GRAPH 
3:RETURN 
To begin data collection, select COLLECT DATA. Once selected the 
screen on the calculator will clear. The program will collect two 
seconds of EKG data. When data has been collected, a graph will 
be displayed and time will be stored in list L1 and voltage 
stored in list L2. To collect more data, press ENTER and select 
YES from the REPEAT menu. To stop data collection and return to 
the MAIN MENU, select NO from the REPEAT menu.
 
The second option in the MAIN MENU, VIEW GRAPH, displays a graph 
of the last data segment collected. This option is helpful when 
it is necessary to take a close look at the graph. To quit from 
this selection and return to the MAIN MENU, press [ENTER] on the 
calculator. 
 
The last option RETURN will exit the program if you are running 
it by itself, or return you to the CHEMBIO MAIN MENU.
XI. OVERVIEW OF EACH PROGRAM 
 
The CHEMBIO program will call the other programs depending upon 
the options you choose. Each program is briefly described below. 
 
CHEMBIO - This is the main program that controls the set up of 
the probes and the experiment. Begin by running this program. 
 
CMBCALIB - This calibration program provides three options 
allowing you to: 1) perform a new calibration, 2) manually enter 
the slope and intercept for a known calibration, or 3) to use a 
default calibration (see CMBCALS). For specific help in 
performing a new calibration, refer to the information sheet that 
came with each sensor. 
 
CMBCALS - This file contains Vernier Software slope and intercept 
calibration values for the various probes. These values will give 
you reasonable data but you may prefer to calibrate each sensor 
individually. Once you have calibrated a specific probe, you may 
want to replace the Vernier calibration values in this file with 
your new values. This file can be edited to contain the new 
values. When you select the USE STORED calibration option, your 
values will then be loaded. Text that is used on the graphs is 
also contained in this program. Note: CHEMBIO must ungrouped 
before you can edit this program with new calibration values.
 
CMBDERIV - This program is used to view graphs of the first and 
second derivative of pH-volume data collected in acid-base 
titration experiments. It assumes that the independent variable, 
volume, is in list L1 and the dependent variable, pH, is in L2. 
When the program is run, the first derivative (delta pH/delta 
volume) is stored in L4 with the corresponding volume in L3. The 
second derivative (delta^2 pH/delta volume^2) is stored in L6 
with the corresponding volume in L5. To take the derivatives, 
exit the CHEMBIO data-collection program and run the CMBDERIV 
program. The program will create three graphs; 1) pH vs. volume, 
2) the first derivative vs. volume, and 3) the second derivative 
vs. volume. 
CMBEKG - This program is run when you choose the EKG sensor from 
the list of probes presented during the probe set up. A complete 
description of the program appears below.
 
CMBGRAPH - The CMBGRAPH program contains graphing routines that 
are used in presenting graphs. 
 
CMBHEART - This program is run when you choose the Heart Rate 
from the list of probes presented during the probe set up. A 
complete description of the program appears below. 
 
CMBMONIT - This program allows you to monitor any or all of the 
three analog channels. Data will be displayed on either the 
calculator or CBL depending upon the combination of probes. 
 
CMBREGRS - This program calculates linear and power regression 
statistics and selected lists. It also allows you to plot a 
linear or power regression curve on a graph.
CMBTIMEG - This program produces graphs of active channels as a 
function of time. Live and non-live displays are produced 
depending upon the combination of probes and length of 
experiment.
 
CMBTRIGP - This program will allow you to collect data such as 
Boyle's law data that may not be time dependent. The program will 
prompt you to input an independent variable such as volume. The 
CBL will then measure a dependent variable such as pressure. 
 
CMBTRIGG - This program uses the [TRIGGER] button on the CBL to 
collect data from each active channel. Each time you press the 
[TRIGGER] button, data are stored in the CBL. At the end of the 
experiment the data are retrieved from the CBL and stored in 
lists on the calculator. 
 
XII. PROGRAM DESIGN NOTES 
 
These programs have been written to support Texas Instruments and 
Vernier probes with the CBL. We hope they help you perform a 
variety of experiments with this exciting technology. Please feel 
free to share these program with other teachers and students. 
Hopefully they have been written in such a way that you can 
modify them for your particular application. 
 
The CMBHEART and CMBEKG programs were designed by Scott Holman 
and written by Matthew Denton. The remainder of the CHEM-BIO 
programs were written by Matthew Denton and Rick Sorensen. The 
programs were designed by Rick Sorensen, Dan Holmquist, Scott 
Holman, and Matthew Denton. Please contact us if you have any 
questions concerning these programs or the use of our probes with 
the programs. 
 
July 22, 1997
 
Rick Sorensen (rsorensen@vernier.com) 
Dan Holmquist (dholmquist@vernier.com)
Scott Holman (sholman@vernier.com) 
Matthew Denton 
Vernier Software 
8565 S.W. Beaverton-Hillsdale Hwy. 
Portland, OR 97225-2429 
phone: (503) 297-5317
fax:   (503) 297-1760
http:\\www.vernier.com
 

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