On Mars, there is a war between the Martian Mice, Plutarkians, and the Sand Raiders. The Plutarkians are fish-humanoids who go around the universe stripping other planets of their resources for their own use. The Sand Raiders are a group of rats, hyennas and other animals that steal and fight with the Plutarkians against the Martian Mice. Among the mice is a group of warriors who call themselves the Freedom Fighters. They fight to bring peace to Mars and to restore its natural resources.
During the biker mice's flight through the cosmos, they were attacked by the Plutarkians, so they crash landed in Chicago's Quigley Field, actually they land in the scoreboard. Needing to make repairs to their bikes, they meet Charlie at the Last Chance Garage. They soon become friends and they now fight the Plutarkian, Laurence Limburger, on earth. "In this wild and wooly universe we live in, there are only three things you can count on: