William Twining born 20 May 1599 in Gloucester England. Died 15 April 1659 in Eastham, Barnstable, Massachusetts. He was married on an unknown date to Ann Doane born 25 July 1666 in Manchester, Lancashire England. She died 12 February 1758 in Eastham, Barnstable, Massachusetts.
Their Children:
i. William Twining born 25 October 1619 in Eastham, Barnstable, Massachusetts. He died 4 November 1703 in Eastham, Barnstable, Massachusetts. He was married about 1650 in Eastham, Barnstable, Massachusetts to Elizabeth Deane born 1629/30 in Plymouth, Massachusetts. She died 28 December 1708 in Newton, Bucks Co., Pennsylvania.
ii. Esabelle Twining born about 1620 in Eastham, Barnstable, Massachusetts.

William Twining and Elizabeth Deane
Their Children:
i. Elizabeth Twining born about 1651 in Massachusetts.
ii. Susanna Twining born 25 January 1653 in Massachusetts.
iii. Joanna Twining born 30 May 1657 in Massachusetts.
iv. Stephen Twining born 6 February 1658/59 in Massachusetts.
v. Mehitable Twining born about 1661 in Massachusetts.
vi. Anne Twining born about 1665 in Massachusetts.
vii. William Twining born 28 February 1667 in Eastham, Barnstable, Massachusetts. He died 23 January 1734 in Eastham, Barnstable, Massachusetts. He was married 26 March 1689 in Eastham, Barnstable, Massachusetts to Ruth Cole born 11 March 1666 in Eastham, Barnstable, Massachusetts. She died 1725/35 in Eastham, Barnstable, Massachusetts.

William Twining and Ruth Cole
Their Children:
i. ElizabethTwining born 25 August 1690 in Eastham, Barnstable, Massachusetts.
ii. Thankful Twining born 11 January 1697 in Eastham, Barnstable, Massachusetts.
iii. Ruth Twining born 27 August 1699 in Eastham, Barnstable, Massachusetts.
iv. Hannah Twining born 2 April 1702 in Eastham, Barnstable, Massachusetts.
v. William Twining born 2 September 1704 in Eastham, Barnstable, Massachusetts.
vi. Barnabas Twining born 29 September 1705 in Eastham, Barnstable, Massachusetts.
vii. Mercy Twining born 20 February 1708 in Eastham, Barnstable, Massachusetts. She was married 15 October 1727 in Eastham, Barnstable, Massachusetts to David Higgins born 5 April 1706 in Eastham, Barnstable, Massachusetts. He died July 1771 in Middle Haddam, Connecticut.

Mercy Twining and David Higgins