N.A.I.A. National Champions
Women's Indoor 400-Meter Dash
| Year | Name | School | Performance |
| 2008 | Marissa Moseley | Oklahoma Baptist | 54.43 |
| 2007 | Patricia Mayers | Wayland Baptist (TX) | 54.60 |
| 2006 | Nickesha Anderson | Missouri Baptist | 54.72 |
| 2005 | Rosemary Okafor | McKendree (IL) | 54.56 |
| 2004 | Niema Golphin | Azusa Pacific (CA) | 54.95 |
| 2003 | Janessa Lepp | Azusa Pacific (CA) | 57.15 |
| 2002 | Sharline Maxwell | Briar Cliff (IA) | 55.55 |
| 2001 | Sharline Maxwell | Briar Cliff (IA) | 56.05 |
| 2000 | Sedell Roberts | Life (GA) | 55.66 |
| 1999 | Zoila Stewart | Life (GA) | 56.43 |
| 1998 | Zoila Stewart | Life (GA) | 55.14 |
| 1997 | Altrice Taylor | Southern-New Orleans | 56.22 |
| 1996 | Fatimat Yusuf | Azusa Pacific (CA) | 53.05 |
| 1995 | Fatimat Yusuf | Azusa Pacific (CA) | 53.24 |
| 1994 | Fatimat Yusuf | Azusa Pacific (CA) | 54.00* |
| 1993 | Fatimat Yusuf | Azusa Pacific (CA) | 54.60* |
| 1992 | Deon Hemmings | Central State (OH) | 56.93* |
| 1991 | Alwren Wallace | Central State (OH) | 56.87* |
| 1990 | Carol Bailey | Concordia (NE) | 56.83* |
| 1989 | Airat Bakare | Azusa Pacific (CA) | 56.88* |
| 1988 | Airat Bakare | Azusa Pacific (CA) | 56.37* |
| 1987 | Denise Bullocks | Chicago State (IL) | 55.68* |
| 1986 | Jocelyn Tatum | Prairie Veiw A&M (TX) | 55.72* |
| 1985 | Carol Williams | Mississippi Valley State | 55.44* |
| 1984 | Margaret Gamble | Prairie View A&M (TX) | 56.30* |
| 1983 | Joyce Townson | Texas Southern | 56.87* |
| 1982 | Loretta Edwards | Prairie View A&M (TX) | 57.32* |
| 1981 | Yvonne Joseph | Jackson State (MS) | 57.96 |
