N.A.I.A. National Champions
Women's Outdoor 400-Meter Dash
| Year | Name | School | Performance |
| 2011 | Verone Chambers | Oklahoma Baptist | 53.43 |
| 2010 | Marissa Moseley | Oklahoma Baptist | 53.54 |
| 2009 | Marissa Moseley | Oklahoma Baptist | 54.02 |
| 2008 | Marissa Moseley | Oklahoma Baptist | 52.87 |
| 2007 | Ruky Abdulai | Simon Fraser (BC) | 52.40 |
| 2006 | Nickesha Anderson | Missouri Baptist | 54.53 |
| 2005 | Nickesha Anderson | Missouri Baptist | 53.39 |
| 2004 | Niema Golphin | Azusa Pacific (CA) | 53.58 |
| 2003 | Crystal Haynes | Webber (FL) | 54.94 |
| 2002 | Daniela Georgieva | Central Methodist (MO) | 53.65 |
| 2001 | Sharline Maxwell | Briar Cliff (IA) | 53.69 |
| 2000 | Sedell Roberts | Life (GA) | 53.77 |
| 1999 | Lucyann Richards | Life (GA) | 53.55 |
| 1998 | Zoila Stewart | Life (GA) | 53.13 |
| 1997 | Zoila Stewart | Life (GA) | 53.34 |
| 1996 | Fatimat Yusuf | Azusa Pacific (CA) | 51.44 |
| 1995 | Beverly Grant | Central State (OH) | 53.13 |
| 1994 | Fatimat Yusuf | Azusa Pacific (CA) | 51.57 |
| 1993 | Audrea Sterling | Central State (OH) | 52.33 |
| 1992 | Audrea Sterling | Central State (OH) | 53.37 |
| 1991 | Audrea Sterling | Central State (OH) | 52.87 |
| 1990 | Rhonda Lampkin | Prairie View A&M (TX) | 54.56 |
| 1989 | Rhonda Lampkin | Prairie View A&M (TX) | 55.20 |
| 1988 | Airat Bakare | Azusa Pacific (CA) | 52.20 |
| 1987 | Denise Bullocks | Chicago State (IL) | 55.21 |
| 1986 | Airat Bakare | Azusa Pacific (CA) | 51.83 |
| 1985 | Carol Williams | Mississippi Valley State | 53.65 |
| 1984 | Denise Bullocks | Chicago State (IL) | 54.09 |
| 1983 | Easter Gabriel | Prairie View A&M (TX) | 53.91 |
| 1982 | Easter Gabriel | Prairie View A&M (TX) | 52.53 |
| 1981 | Loretta Edwards | Prairie View A&M (TX) | 54.38 |
