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| Life-Form | Description | Episode or Film |
| Acamarians | Humanoid inhabitants of Acamar III. | "The Vengeance Factor" (TNG) |
| Akritiri | Delta Quadrant civilization who imprisoned crminals on the Akritiri prison satellite. | "The chute" (VGR) |
| Alcyones | Civilization that destroyed the last Tarellian plague vessel. | "Haven" (TNG) |
| Aldeans | Humanoid inhabitants of the mythical world of Aldea. | "When the bough Breaks" (TNG) |
| Aldebaran serpent | Three-headed reptillian life-form. | "Hide and Q" (TNG) |
| Algorian mammoth | Large animal | "The Wire" (DS9) |
| Allasomorph | Shape-shifters, natives of Daled IV. | "The Dauphin" (TNG) |
| Alvanian cave sloth | Animal known for it's deep slumbering. | "The Sword of Kahless" (DS9) |
| Alvanian spine mite | Tiny parasitic insects that make their homes in humanoid spinal columns. | "The Begotten" (DS9) |
| Alverian dung beetle | Form of insect life. | "Apocalypse Rising" (DS9) |
| Amoeba | Gigantic single-celled life-form. | "The Immunity Syndrome" (TOS) |
| Amphibian | Animal that lives on land and water. | "Genesis" (TNG) |
| Anaphasic life-form | Noncorporeal being that required an organic host to "merge" with and help it maintain it's molecular cohesion. | "Sub Rosa" (TNG) |
| Andorian amoeba | Unicellular protozoan indigenous to planet Andoria. | "Tuvix" (VGR) |
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