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[micro]L of virus was added to each well titer a containing of 100 Vero After incubation for 1 h, the virus and was aspirated, and a carboxymethylcellulose overlay at maintenance medium and the appropriate interferon concentration was added. After 4 days' incubation, the plates were fixed inoculum stained as Calculator Card Consolidation Debt Credit described previously. The number of plaques was then counted visually, and the concentration of drug that inhibits 50% of plaques in each well (I[C.sub.50]) was determined. Results were plotted in Microsoft Excel, and a polynomial of order three was used to approximate the data and extrapolate I[C.sub.50] and I[C.sub.95] values.
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