U.S. Federal Route 65 runs briefly through the northwestern corner of Jasper County. From the Polk/Jasper County Line to Iowa State Route 117/330, this is a four lane divided highway with at-grade intersections. From IA St Rt 117/330 to the Jasper/Story County Line, it is a two-lane highway. U.S. Rt. 65 does not pass through any towns in Jasper County. U.S. Rt. 65 is not multi-plexed in Jasper County. U.S. Rt. 65 intersects the following highways in Jasper County. Iowa State Route 117, near Mingo. Iowa State Route 330, near Mingo.
A junction marker on NB IA St Rt 117. A guide marker on NB IA St Rt 117. A junction marker on SB IA St Rt 330. Another junction marker on SB IA St Rt 330. A guide marker on SB IA St Rt 330. NB U.S. Rt. 65 north of IA St Rt 117/330. A street marker in Jasper County.
