Iowa State Route 27 runs along Interstate 35 the entire length of Worth County. This runs through the western parts of the county. It runs from the Iowa/Minnesota State Line to the Worth/Cerro Gordo County Line. IA St Rt 27 does not pass through any towns in Worth County. IA St Rt 27 is multi-plexed in Worth County. Interstate 35, in Worth County.
IA St Rt 27 intersects the following highway in Worth County. Iowa State Route 9, near Hanlontown.
NB I-35/IA St Rt 27 near the Minnesota/Iowa State Line.