Biochemistry Topics                                                                            


(1) Introduction
Cells, Biomolecules and Water; Amino Acids; Polypeptides
(2) Proteins
Structure; Separation and Purification; Allosteric Effects
(3) Enzymes
Coenzymes; Kinetics; Mechanisms; Regulation

(4) Membranes
Lipids; Structure and Function; Transport and Signaling
(5) Bioenergetics
Free Energy of Reaction; Carbohydrates and Uptake
(6) Glycolysis
Main and
Alternative Pathways; Inhibitors; Regulation

(7) Citric Acid Cycle
Pyruvate; Reducing Equivalents; ATP Yield; Regulation; Inhibitors
(8) Oxidative Phosphorylation
Mitochondrial Transport; Electron Transfer Chain; Uncoupling
(9) Pentose Phosphate Pathway Oxidative and Non-oxidative; NAPDH; Regulation

(10) Carbohydrate Synthesis
Gluconeogenesis;
Glycogen Synthesis and Degradation
(11) Protein Metabolism
Zymogens; Absorption and Degradation; Urea Cycle
(12) Lipid Metabolism
Fatty Acid Synthesis
; Fatty Acid Oxidation; Cholesterol

(13) Integration of Metabolism
ATP/ADP Cycle; Control Sites; Starvation vs. Feeding
(14) Nucleotides
Structure;
Purine Metabolism; Pyrimidine Metabolism
(15) DNA Replication
DNA and RNA Properties; DNA Polymerases; Replication Fork

(16) Transcription
Prokariotic; Eukariotic; RNA Synthesis; Regulation
(17) Protein Synthesis
Codons; tRNA; Ribosomes; Eukaryotic vs. Prokaryotic
(18) Recombinant DNA
Cloning, Sequencing, Genomic Libraries, PCR

FINAL EXAM