Pharmacology and Toxicology Quizzes  

G-proteins Quiz 3

1- Subunits of heterotrimeric G-protein
alpha, beta and gamma
alpha and beta/gamma
Ras, GNEF and GAP
none of the above


2- Contains intrinsic GTPase activity
alpha subunit
beta/gamma complex
GNEF
GAP


3- Approximate size of gamma subunit
8 to 10 kD
20 to 30 kD
35 to 36 kD
39 to 46 kD


4- Nucleotide exchange in a heterotrimeric G-protein is catalyzed by
activated receptor
alpha subunit
GAP
GNEF


5- A G-protein participates in transduction if
resistant analogs of GTP weaken ligand binding
agonist binding is stimulated by GTP
either pertussin toxin or cholera toxin block the effect
all of the above


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Answers:

1- Subunits of heterotrimeric G-protein
Answer: alpha, beta and gamma


2- Contains intrinsic GTPase activity
Answer: alpha subunit


3- Approximate size of gamma subunit
Answer: 8 to 10 kD


4- Nucleotide exchange in a heterotrimeric G-protein is catalyzed by
Answer: activated receptor


5- A G-protein participates in transduction if
Answer: resistant analogs of GTP weaken ligand binding