Basic Physics of pH and Conductivity
More on NK Cells
"[pH]ex 7.3-7.6"
Accession No.: 96101983.
Author: Radosevi'c-K. de-Grooth-B-G. Greve-J.
Title: Changes in intracellular calcium concentration and pH of
target cells during the cytotoxic process: a quantitative study
at the single cell level.
Source: Cytometry. 1995 Aug 1. 20(4). P 281-9.
Journal Title: CYTOMETRY.
Abstract:
This study reports on the changes in intracellular calcium
concentration ([Ca2+]in) and intracellular pH ([pH]in) that
occur in K562 target cells during interaction with human Natural
Killer (NK) cells. The data were obtained using a quantitative
fluorescence microscope and fluorescent ratio probes specific
for [Ca2+]in (Fura-2-AM) and [pH]in (BCECF-AM). Results
demonstrate that two types of target cell response to the attack
by an NK cell can be distinguished. The target cell either dies
immediately, due to the complete breakdown of the membrane
impermeability, or the initial membrane damage
(i.e., increasedmembrane permeability) is
repaired and the cell "escapes" immediate death. During both
responses an increase of [Ca2+]in takes place in the target
cells. In the cells that die immediately, however, [Ca2+]in
reaches higher levels (approximately 1,400 nM) than in the
cells that restore the initial damage (approximately 700 nM).
Changes in target cell [pH]in are also detected during both
responses. The direction of the change (acidification or
alkalinization) as well as the level of the change depend on
extracellular pH ([pH]ex). Also, [pH]in remains changed during
the time the cells were followed (10 min). The programming time
(i.e., the time from the initiation of the cytotoxic process to
the time that a change in the physiological parameter was
detected) of the killing process that leads to an immediate
target cell death appears to be shortest at [pH]ex 7.3-7.6
(approximately 3 min).
Holdings: Health Sciences Serials
SHELVED BY TITLE: Cytometry
CALL NUMBER: QH573 .C95
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