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Dec. 2000

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March 2000 June 2000 Sept. 2000 Dec. 2000

Journal of Applied Animal Research

Vol.18 No. 2           December, 2000

(Abstracted/indexed in AGRIS Database, Biosis Database, CAB Abstracts,Chemical Abstracts, CURRENT CONTENTS (AB and ES), Food Science and Technology Abstracts, Indian Science Abstracts, RESEARCH ALERT, SCISEARCH)

TOPIC AUTHOR

Variation in egg content density makes egg specific gravity a poor indicator of shell weight

D.R. Sloan, R.H. Harms, A.G. Abdullah and K.K. Kuchinski (USA)

Involvement of central gastrin and cholecystokinin in the regulation of food intake in the neonatal chick

M. Furuse, T. Bungo, R. Ao, R. Ando, M. Shimojo, Y. Masuda (Japan) and
D.M. Denbow (USA)

Effect of laidlomycin propionate on beef bull growth performance and reproductive development

K.M. Downs, W.E. Kunkle, T.T. Marshall, B.A. Reiling and J.V. Yelich (USA)

Rhodococcus equi infection in goat : Apropos of two cases

J.L. Rodriguez, B. Acosta, R. Navarro and C. Gutierrez (Spain)

Anatomy and histology of the sinu-atrial node of goats (Capra hircus)

A. Nabipur, S. Khanzadi and G.H. Moradi (Iran)

Field peas (Pisum sativum) as protein source in diets of growing-finishing pigs

T.S. Brand, D.A. Brandt, J.P. van der Merwe and
C.W. Cruywagen (South Africa)

Contribution of small ruminants (sheep and goats) to meat supply in Enugu state, Nigeria

B.I. Odo, S.O. Alaku and S.I. Omeje (Nigeria)

Effect of Isoflupredone acetate (Predef. 2.X) on ovulation and oestrous cycle in mare

M.R. Ahmadi, M. Kafi and A.Kh. Aghil (Iran)

Ascending phase lactation characteristics of Red Sokoto goats

G.N. Akpa, O.E. Asiribo and O.O. Oni (Nigeria)

Effect of dietary halophyte Salicornia bigelovii Torr on carcass characteristics, minerals, fatty acids and amino acids profile of camel meat

A.N. Al-Owaimer (Saudi Arabia)

Effect of roughage type and duration of nutrient restriction on compensatory growth of crossbred bulls

Tesfaye Alemu Aredo (Ethiopia)

Erythrocyte membrane sialoglycoproteins. I. Qualitative and quantitative differences in Zebu and Taurine cattle and buffalo

V.O. Taiwo and A.O. Ogunsanmi (Nigeria)

Response of different dietary energy and protein combinations on growth performance of broiler starters

Ashenafi Mengistu and Alemu Yami (Ethiopia)

Ultrastructural observations on bufallo parotid gland

S.H. Mansouri and J. Mehrabi (Iran)

Effect of gastro-intestinal parasitosis on tuberculin test for the diagnosis of bovine tuberculosis

Gobena Ameni and Girmay Medhin (Ethiopia)

Production and efficacy of Peste des Petits ruminants vaccine 75/1 in the Kingdom of Saudi Arabia

S.M. Rashwan, H.N. Al-Khalaf, M.I. Al-Hammad, A.H. Azab, M.H. Moustafa and A. Diallo (Saudi Arabia)

Variation in Egg Content Density Makes Egg Specific Gravity a Poor Indicator
of Shell Weight1

D.R. Sloan2, R.H. Harms, A.G. Abdullah, K.K. Kuchinski

Department of Dairy and Poultry Sciences
University of Florida
Gainesville, Florida 32611-0930 USA

(Received January 31, 2000; accepted August 16, 2000)

Abstract

Sloan, D.R., Harms, R.H., Abdullah, A.G. and Kuchinski, K.K. 2000. Variation in egg content density makes egg specific gravity a poor indicator of shell weight. J. Appl. Anim. Res., 18: 121-128.

A study was conducted to measure the variability in egg content specific gravity (ECSG) among breeds and strains, and ages of laying hens. Also information is presented concerning how the specific gravity of egg content (EC) affects the specific gravity of egg (ESG). The observed ECSG ranged from a low of 1.0298 to a high of 1.0352. Based on the formula of Harms et al. (1990) a change of ECSG from 1.029 to 1.035 would change the ESG from 1.0823 to 1.0883 and the eggshell weight from 5.64 to 6.18 g. ECSG on a commercial farm ranged from 1.0323 to 1.0352. This resulted in calculated ESG from 1.0856 to 1.0885 and calculated SW from 5.84 to 6.21g. Researchers and industrymen must be very careful in using ESG data in making decisions relating to eggshell quality.

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Involvement of Central Gastrin and Cholecystokinin in the Regulation of Food Intake in the Neonatal Chick

M. Furuse*, T. Bungo, R. Ao, R. Ando, M. Shimojo,
Y. Masuda, D.M. Denbow1

Division of Animal and Marine Bioresources Science
Graduate School of Bioresource and Bioenvironmental Sciences
Kyushu University, Fukuoka 812-8581, Japan

1Department of Animal and Poultry Science
Virginia Polytechnic Institute and State University
Blacksburg, VA 24061, USA

(Received April 11, 2000; accepted September 26, 2000)

Abstract

Furuse, M., Bungo, T., Ao, R., Ando, R., Shimojo, M., Masuda, Y. and Denbow, D.M. 2000. Involvement of central gastrin and cholecystokinin in the regulation of food intake in the neonatal chick. J. Appl. Anim. Res., 18: 129-136.

The effects of intracerebroventricular (ICV) injection of chicken gastrin and cholecystokinin (CCK) on food intake were compared in the neonatal chick. In Experiment 1, the effect of 131 pmol of chicken gastrin (36 amino acid residues) on food intake was compared with 66 and 131 pmol of CCK-8S over 2h. Chicken gastrin strongly inhibited food intake, but both levels of CCK-8S did not. The similar effect of 131 pmol chicken gastrin was obtained by 262 pmol CCK-8S at 1h after ICV injection in Experiment 2. This suppressive effect of chicken gastrin continued over 2h after ICV injection, though the effect of CCK-8S had disappeared by 2h. In Experiment 3, the effects of 131 pmol of CCK-8S, CCK-33S and chicken gastrin were compared. The effect of CCK-8S was weak, but CCK-33S and chicken gastrin similarly and strongly inhibited food intake of chicks. These results suggested that the suppressive effect of food intake of gastrin/CCK family may be dependent upon the length of amino acid sequence.

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Effect of Laidlomycin Propionate on Beef Bull Growth Performance and Reproductive Development1

K.M. Downs2, W.E. Kunkle, T.T. Marshall,
B.A. Reiling, J.V. Yelich

Animal Science Department
University of Florida
Gainesville, Florida 32611-0910, USA

(Received April 18, 2000; accepted June 15, 2000)

Abstract

Downs, K.M., Kunkle, W.E., Marshall, T.T., Reiling, B.A. and Yelich, J.V. 2000. Effect of laidlomycin propionate on beef bull growth performance and reproductive development. J. Appl. Anim. Res., 18 : 137-147.

The effects of laidlomycin propionate (LP) on beef bull growth performance and sexual development were determined in an 84 day study. Forty embryo transfer Angus bulls were fed three complete diets containing LP levels of either 0, 10 or 20 g/t. Bulls were fed daily using electronic feeders to obtain individual feed intake. Full weight and scrotal circumference (SC) were evaluated at 28 day intervals. Breeding soundness examinations were conducted to characterize semen. Muscle mass and body fat were estimated using real-time ultrasonography. Laidlomycin propionate did not influence daily gain, dry matter intake, feed efficiency, subcutaneous or intramuscular fat deposition, but Longissimmus muscle deposition was enhanced. Among treatments, bulls had similar (P>0.05) SC measurements and no detrimental effects on semen quality or sperm morphology were observed. Supplementing growing bulls with laidlomycin propionate for improved performance did not detrimentally affect sexual development.
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Rhodococcus equi Infection in Goat: Apropos of Two Cases

J.L. Rodriguez, B. Acosta, R. Navarro*,
C. Gutierrez1

Department of Animal Science, Facultad de Veterinaria
Universidad de Las Palmas de Gran Canaria
35416 Las Palmas, Canary Islands, Spain

(Received November 2, 1999; accepted July 3, 2000)

Abstract

Rodriguez, J.L., Acosta, B., Navarro, R. and Gutierrez, C. 2000. Rhodococcus equi infection in goat: Apropos of two cases. J. Appl. Anim. Res., 18: 149-151.

Two cases of Rhodococcus equi infection in Spanish goats have been described. Clinical signs were chronic weight loss, agalactia, fever, prone position and apparent abdominal pain in one of them. Multifocal well encapsulated scattered abscess were observed in liver. Stress, sanitary deficiencies and moderate cold environment few months ago could have caused the establishment of infection.
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Anatomy and Histology of the Sinu-Atrial Node of Goats (Capra hircus)

A. Nabipur, S. Khanzadi, G.H. Moradi

Department of Anatomical Sciences
School of Veterinary Medicine
Ferdowsi University of Mashhad, Mashhad, 91775-1793, Iran

(Revised received April 3, 2000; accepted June 15, 2000)

Abstract

Nabipur, A., Khanzadi, S. and Moradi, G.H. 2000. Anatomy and histology of the sinu-atrial node of goats (Capra hircus). J. Appl. Anim. Res., 18 : 153-158.

Anatomy of the sinu-atrial node was studied in 5 female goats. The sinu-atrial node(12.75mm × 1.5mm × 1.7mm) lied 0.18mm-0.25mm beneath the epicardium, at terminal sulcus, near the junction between the cranial vena cava, lateral wall of the right atrium and the right auricle. Its shape was elongated and relatively cone shaped. Its cranial end was near the myocardium of atrial wall. Histologically, it normally contained a dense collagen frame and the cells with clear perinuclear zone (P) and transitional cells (T) were the principal cells of parenchyma. The "P" cells were bigger than "T" cells and smaller than the ordinary myocardial cells. At the junction between "T" cells and "P" cells intercalated discs were not seen. Near the central section and toward the cranial margin there is a distinct arteriole that supplies the node.
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Field Peas (Pisum sativum) as Protein Source in Diets of Growing-Finishing Pigs

T.S. Brand1, D.A. Brandt2, J.P. van der Merwe1,
C.W. Cruywagen2

1Elsenburg Agricultural Research Centre
Private Bag XI, Elsenburg 7607, South Africa

2Department of Animal Science
Faculty of Agricultural Sciences, University of Stellenbosch
Private Bag XI, Matieland 7602, South Africa

(Received on December 21, 1999; accepted August 3, 2000)

Abstract

Brand, T.S., Brandt, D.A., van der Merwe, J.P. and Cruywagen, C.W. 2000. Field peas (Pisum sativum) as protein source in diets of growing-finishing pigs. J. Appl. Anim. Res., 18: 159-164.

The field pea (Pisum sativum) cultivar Glenroy (187 g/kg CP) was evaluated as an alternative protein source in diets of grower-finisher pigs. Four diets were formulated on iso-nutrient basis (»14.4 MJ/kg DE, 160 g/kg CP, 9.3 g/kg lysine, 5.3 g/kg methionine and cystine, 2.1 g/kg tryptophan) so that soybean oilcake meal and maize meal were substituted by increasing levels of fieldpeas (16%, 32%, 48% and 64%). Forty castrated pigs (78 d, 21.7±2.6 kg) were individually housed and 10 pigs were fed on each diet ad libitum for 84 days. DM intake, average daily gain and feed conversion efficiency, all depressed on the highest peas level in the diet. The DE content and N-retention of the 64% peas diet were significantly (P<0.05) lower than those of the 16% and 32% pea diets. Though dressing percentage was not affected by dietary treatment, P2 back fat thickness was significantly decreased in the 64% peas inclusion group resulting in leaner carcasses. This was mainly due to lower slaughter weights. It was concluded that up to 32% of the field pea cultivar Glenroy in the diets of growing pigs can be recommended.
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Contribution of Small Ruminants (Sheep and Goats) to Meat Supply in Enugu State, Nigeria

B.I. Odo, S.O. Alaku, S.I. Omeje

Department of Animal Science
Enugu State University of Science and Technology
P.M.B. 053, Abakaliki Campus
Abakaliki, Nigeria

(Revised received June 6, 2000; accepted August 30, 2000)

Abstract

Odo, B.I., Alaku, S.O. and Omeje, S.I. 2000. Contribution of small ruminants (sheep and goats) to meat supply in Enugu state, Nigeria. J. Appl. Anim. Res., 18: 165-169.

The monthly records of various livestock species (cattle, sheep, goat, pig, horse, donkey and dog) slaughtered for meat in Enugu state for a period of 8 years (1988-1995) were used to determine the contribution of small ruminants to meat supply in the State. The contribution made by the small ruminants was lowest (16.13%) in 1991 and highest (24.62%) in 1993 with an average contribution of 20.40±4.02% during the 8 years period of survey. Most small ruminants were slaughtered in the Enugu zone of the state. This study confirms the usefulness of sheep and goats in animal protein supply in the state.
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Effect of Isoflupredone Acetate (Predef. 2X) on Ovulation and Oestrous Cycle in Mare

M.R. Ahmadi, M. Kafi, A.Kh. Aghil

Department of Clinical Sciences
School of Veterinary Medicine
Shiraz University, Shiraz 71345-1731, Iran

(Received October 20, 1999; accepted September 10, 2000)

Abstract

Ahmadi, M.R., Kafi, M. and Aghil, A. Kh. 2000. Effect of Isoflupredone acetate (Predef. 2X) on ovulation and oestrous cycle in mare. J. Appl. Anim. Res., 18: 171-175.

To evaluate the effect of Isoflupredone acetate (Predef. 2x) on ovulation and the length of the oestrus cycle in the mare, six non-pregnant reproductively sound mix-breed mares were used in this study. The first oestrus cycles of the mares were monitored and considered as control oestrus cycles. Oestrus cycles of the mares were synchronized using a dose of prostaglandin F2a (Lutalyse, 10 mg/mare) and hCG (3000 I.U./mare). Each mare was injected intramuscularly 20 mg of Isoflupredone acetate from day 10 of the oestrous cycle up to the day of ovulation. Ovulation occured in all mares 24 to 48h after hCG treatment. There was no significant difference in the length of the oestrus cycle, the duration of the follicular and luteal phases between the control and Isoflupredone acetate treated oestrus cycles. It is concluded that there was no detrimental effect of Isoflupredone acetate on the length of oestrus cycle and ovulation in the mare.
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Ascending Phase Lactation Characteristics of Red Sokoto Goats

G.N. Akpa, O.E. Asiribo1 , O.O. Oni2

Animal Science Department
Ahmadu Bello University, Zaria, Nigeria

(Received February 22, 2000; accepted October 3, 2000)

Abstract

Akpa, G.N., Asiribo, O.E. and Oni, O.O. 2000. Ascending phase lactation characteristics of red sokoto goats. J. Appl. Anim. Res., 18: 177-184.

The ascending phase milk yield characteristics were evaluated in Red Sokoto goats using 120 days milk yield records obtained from a total of 92 lactating does in 6 herds, collected over a two year period. The ascending phase characteristics were highly variable (47.6-74.5%) and were significantly (P<0.01-0.05) influenced by herd, season and parity, but not by litter size and litter composition (P>0.05). Highly positive and significant (P<0.01) correlations (r=0.62) exist between total yield and ascending phase total and average day yield, respectively. Negative and significant (P<0.05) correlation (r=-0.30) was observed for peak week and days in the ascending phase. The significant herd and seasonal effects may have practical implications in determining optimal feeding management and season of breeding to maximize total lactation yield. The effect of parity point to the fact that it is not necessary to cull lactating does before parity 5.

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Effect of Dietary Halophyte Salicornia bigelovii Torr on Carcass Characteristics, Minerals, Fatty Acids and Amino Acids Profile of Camel Meat

A.N. Al-Owaimer

Department of Animal Production
College of Agriculture
King Saud University, P.O. Box 2460
Riyadh 11451, Saudi Arabia

(Received May 10, 2000; acceptance October 3, 2000)

Abstract

Al-Owaimer, A. N. 2001. Effect of dietary halophyte Salicornia bigelovii Torr on carcass chacracteristics, minerals, fatty acids and amino acids profile of camel meat. J. Appl. Anim.Res., 18: 185-192.

To evaluate the effect of feeding Salicornia bigelovii, a halophylte, eleven Najdi camels (202 kg) were divided into two groups and fed on diets containing either 25% Rhodes grass or Salicornia hay for 120 days. Carcass characteristics were generally not affected by Salicornia hay. Similarly proximate composition, minerals, essential amino acids and fatty acids of M. longissimus were generally not affected by dietary treatments. However, Na, linoleic acid, Polyunsaturated fatty acids and methionine were higher in Salicornia hay fed camels, whereas, essential amino acid valine, histidine and phenyl alanine were lower in these camels. It is concluded that Salicornia hay can be included at 25% level in the diet of adult Najdi camels.

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Effect of Roughage Type and Duration of Nutrient Restriction on Compensatory Growth of Crossbred Bulls

Tesfaye Alemu Aredo

Adami Tulu Research Center
P.O.Box 35, Zeway, Ethiopia

(Revised received September 30, 2000; accepted October 6, 2000)

Abstract

Aredo, T.A. 2000. Effect of roughage type and duration of nutrient restriction on compensatory growth of crossbred bulls. J. Appl. Anim. Res., 18: 193-198.

The effect of nutrient restriction imposed by feeding poor quality roughage (hay for 6 months and maize stover for 6 or 3 months) and subsequent replenishment through concentrate feeding (3 kg/h/d for 120 days) on compensatory growth was studied using 18 crossbred (5/8 Friesian, 3/8 Zebu) bulls ranging in age from 1 to 2.5 years. During the restriction period, animals on hay gained 1.3 kg live weight per head, whereas those on maize stover for 6 months lost 22.8 kg per head. The difference in weight gains of animals restrictedly fed on maize stover for 3 and 6 months was not significant (P>0.05). At the end of the experimental period, body weight gain of the animals was not affected (P>0.05) both by the feed types and the duration of nutrients restriction. Feed cost per kg of weight gained was significantly (P<0.05) lower for animals fed on maize stover for 6 months compared to those fed on hay for the same duration. It is concluded that in areas where maize stover is available and livestock feed is scarce, especially during the dry season, animals may be fed on maize stover for 6 months followed by a realimentation period of about 4 months.

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Erythrocyte Membrane Sialoglycoproteins. I. Qualitative and Quantitative Differences in Zebu and Taurine Cattle and Buffalo

V.O. Taiwo, A.O. Ogunsanmi1

Department of Veterinary Pathology
1Department of Wildlife and Fisheries Management
University of Ibadan, Ibadan, Nigeria

(Revised received September 15, 2000; accepted September 18, 2000)

Abstract

Taiwo, V.O. and Ogunsanmi, A.O. 2000. Erythrocyte membrane sialoglycoproteins. I. Qualitative and quantitative differences in zebu and taurine cattle and buffalo. J. Appl. Anim. Res., 18: 19-208.

Comparative assays on the concentration and types of sialoglycoproteins (glycophorins; GP) on the erythrocyte membrane of three different cattle breeds and buffalo were carried out. Twenty-eight cattle consisting of ten N'Dama (Bos taurus), fourteen White Fulani, (West African Zebu; WAZ), four Boran (East African Zebu; EAZ) and two buffaloes were used. The erythrocytes of WAZ and EAZ cattle contained significantly lower (P<0.05) concentrations of sialoglycoproteins (9.85±0.61 and 9.46±1.35 mg/109 erythrocytes, respectively) than those of N'Dama cattle (Ibadan-based, 12.54±0.37; Nairobi-based, 12.28±0.85 mg/109 erythrocytes) and buffalo (10.43±1.25 mg/109 erythrocytes). Electrophoretic separation of the erythrocyte GPs revealed that both N'Dama cattle, its 75% crosses and buffalo expressed three bands corresponding to human erythrocyte GPA, GPC and GPB, with 39, 30 and 25 kDa apparent molecular masses, respectively. On the other hand, WAZ cattle expressed GPA and GPC while EAZ cattle erythrocytes expressed only the GPA band. These findings indicate that the N'Dama cattle and buffalo, both of which are known to be trypanotolerant, have qualitatively and quantitatively higher levels and types of erythrocyte membrane glycophorins than the trypanosusceptible WAZ and EAZ cattle. It is concluded that these erythrocyte membrane attributes of N'Dama and buffalo may be heritable characteristics and possible markers for trypanotolerance.

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Response of Different Dietary Energy and Protein Combinations on Growth Performance of Broiler Starters

Ashenafi Mengistu1, Alemu Yami2

1Adami Tulu Research Center
P.O. Box 35, Ziwa, Ethiopia

2Debre Zeit Research Center
P.O. Box 32, Debre Zeit, Ethiopia

(Received October 25, 1999; accepted August 16, 2000)

Abstract

Mengistu, A. and Yami, A. 2000. Response of different dietary energy and protein combinations on growth performance of broiler starters. J. Appl. Anim. Res., 18: 209-214.

A 4x4 factorial experiment in a completely randomized design using four dietary levels of energy (2600, 2700, 2800 and 2900 kcal ME/kg) and four of protein (18, 20, 22 and 24 percent CP) was conducted at the Debre Zeit Agricultural Research Center to study the effect of different energy and protein combinations of starter diets on growth performance of broilers. Increasing the protein level of broiler starter diets favours higher feed intake with a subsequent improvement in growth performance.
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Ultrastructural Observations on Buffalo Parotid Gland

S.H. Mansouri, J. Mehrabi

Department of Anatomical Sciences
School of Veterinary Medicine
Shiraz University, Shiraz, 71345-1144, Iran

(Received February 7, 2000; accepted September 13, 2000)

Abstract

Mansouri, S.H. and Mehrabi, J. 2000. Ultrastructural observations on bufallo parotid gland. J. Appl. Anim. Res., 18: 215-220.

The fine structure of the parotid gland of buffalo was studied by electron microscopy. The parenchyma of the gland consisted of compound tubulo-alveolo-acinar serous secretory units, intercalated ducts, nonstriated intralobular ducts and excretory ducts. The secretory units were mostly tortuous and lined with cells of variable heights, giving a scalloped appearance to secretory lumina. Secretory cells contained rough endoplasmic reticulum, Golgi apparatus and numerous mitochondria with dark granules. A few electron-dense secretory granules of different sizes were also found in the apical cytoplasm of secretory cells. Unlike other ruminants, the luminal surface of the secretory units and intercellular canaliculi were devoid of any microvilli. Secretory granules were not found in the cytoplasm of intercalated and nonstriated intralobular duct cells.

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Effect of Gastro-intestinal Parasitosis on Tuberculin Test for the Diagnosis of
Bovine Tuberculosis

Gobena Ameni1,2*, Girmay Medhin1

1Institute of Pathobiology
2Faculty of Veterinary Medicine
Addis Ababa University, P.O. Box 1176, Ethiopia

(Received March 8, 2000, accepted September 16, 2000)

Abstract

Ameni, G. and Medhin, G. 2000. Effect of gastro-intestinal parasitosis on tuberculin test for the diagnosis of bovine tuberculosis. J. Appl. Anim. Res., 18: 221-224.

A study was conducted on hundred-one heifers aging 1.5 to 2.5 years to determine the effect of infection with different gastro-intestinal parasites namely, fasciola, paramphistomum, schistosome, strongyle and coccidia on the immune response to tuberculin test. De-wormed heifers reacted to tuberculin injection significantly differently (P<0.05) than non de-wormed ones. Significantly (P<0.05) higher immune response to tuberculin injection was observed when animal was negative than when it was positive for fasciola or strongyle only. This preliminary work warrants further investigation on the importance of de-worming animals before conducting tuberculin test for the diagnosis of bovine tuberculosis.
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Production and Efficacy of Peste Des Petits Ruminants Vaccine 75/1 in the Kingdom of Saudi Arabia

S.M. Rashwan, H.N. Al-Khalaf, M.I. Al-Hammad,
A.H. Azab, M.H. Moustafa, A. Diallo

Veterinary Vaccine Production Center (VVPC)
Riyadh 11454-15831, Kingdom of Saudi Arabia

(Received April 28, 2000; accepted September 27, 2000)

Abstract

Rashwan, S. M., Al-Khalaf, H. N., Al-Hammad, M. I., Azab, A. H., Moustafa, M. H. and Diallo, A. 2000. Production and efficacy of peste des petits ruminants vaccine 75/1 in the Kingdom of Saudi Arabia. J. Appl. Anim. Res., 8: 225-231.

A freeze dried live attenuated vaccine against peste des petits ruminants (PPR) infection was prepared by growing an attenuated PPR strain 75/1 in vero cell culture. The produced vaccine batch that had a titre of 2x6.3 log 10 TCID50/100 dose was safe and potent. The mean titre of PPRV-neutralizing antibodies was 2.2 log 10 at the 21st day post-vaccination. The vaccine was thermostable when stored at 4C for 360 days. The reconstituted vaccine was still within the recommended titre after keeping at 20C for 8 hours, 37C for 4 hours and 45C for 1 hour.

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